• 微量生物(大小分子)分析化學
• 毛細管電泳技術

• 謝明穆助理教授

• 於輔英科技大學這幾年來除了積極架設自己研究設備外（目前毛細管電泳UV和LIF的偵測系統各一套），其研究主軸則著重於微量化學與生物樣品的分析，實驗的架設依照不同樣品的特性以毛細管電泳發展出不同分析檢測技術。主要區分為三種研究如小分子的研究、大分子的研究及奈米粒子研究。小分子的研究方面，利用自組LIF毛細管設備獨自開發出使用胺和鹵素離子形成離子對而改變分析物電淌度之觀念，來提升分離生物胺的解析度與靈敏度(偵測極限可達nM)。除此之外也使用階段式的溶液概念獨自發展出濃縮與分離神經傳導物的技術，此技術不但可以同時用來分離兒茶酚胺與代謝酸化合物，而且偵測極限可達pM [2]，因無須繁雜樣品處理步驟，這些開發的方法具有低成本、簡單和易自動化等優點。另一方面也尋求校內同事的合作，共同發展出分析天然物（香椿）的組成的技術，目前已接受一篇，另一篇審稿中。大分子的研究方面，最近也發展出另一種新的線上濃縮蛋白質的檢測技術，此技術除了使用高靈敏度雷射誘導螢光偵測，並利用低濃度的SDS或者PEO來準備蛋白質的樣品，加上搭配SDS的溶液區段，使得原本難以偵測的蛋白質的濃度都可以輕易被檢測出，如以UV-Vis的偵測方式也都有不錯的結果，在奈米粒子研究方面，承緒與同仁合作，成功開發於水相溶液中合成出會發出不同顏色螢光的量子點（CdSe和CdSe@CdS），此外在高功率光照射下，探討蛋白質及溶液酸鹼值對合成大小與顏色不同之金奈米粒子之影響，並在適當條件下，合成穩定及較純之球狀金奈米粒子。最後綜合這些結果完成兩篇相關於毛細管電泳分析大小分子與奈米粒子回顧文獻。

• 提升毛細管電泳分析生物胺與酸之偵測靈敏度(1/2)。


• 提升毛細管電泳分析生物胺與酸之偵測靈敏度(2/2)。


• 以毛細管電泳配合發光二極體誘導螢光分析神經傳導物質。
• 以毛細管電泳配合發光二極體誘導螢光分析茶飲料及茶葉中之胺基酸。

A、期刊發表

1. Ming- Mu Hsieh, Huan-Tsung Chang* ,“Dynamic control the separation of organic acids in the capillary electrophoresis”, J. Chromatogr. .A. 793 (1998) 145-152. (SCI)
   public JAN
2. Ming-Mu Hsieh, Huan- Tsung Chang*, “ Dynamic control for ultra-fast separations?? of organic acids in capillary zone electrophoresis a new direction to improvement resolution”, J. Chromatogr. A. 817 (1998) 129-137. (SCI) public AUG
3. 張煥宗，謝明穆，曾韋龍 “雷射激發螢光偵測器於生物分析中之應用” 光訊 76 (1999) 20-23.
4. Huan- Tsung Chang*, Hsuan- Shen Chen, Ming- Mu Hsieh and Wei-Lung Tseng, “ Electrophoresis separation of DNA in the presence of electrophoresis Flow”, Review in Analytical Chemistry 19 (2000) 46-74. (SCI)
5. Ming- Mu Hsieh, Wei-Lung Tseng, Huan-Tsung Chang* , “ On-column? preconcentration and separation of DNA fragments using polymer solution in the presence of electrophoresis of electroosmotic flow”, Electrophoresis 21 (2000) 2904-2910. ?(SCI)? public AUG
6. Wei-Lung Tseng, Ming-Mu Hsieh, Shang-Ji Wang, Huan- Tsung Chang*, “Effect of ionic strength, pH and polymer concentration on the separation of DNA fragments in the presence of electroosmotic flow.”,? J. Chromatogr. A. 894 (2000) 219-230. (SCI) public OCT
7. Ming-Mu Hsieh, Yi-Chun Kuo, Myan-jen Lyu, Huan-Tsung Chang*, “ Dynamic modification of the capillary wall for electrophoresis separation of small ions”, J. Chromatogr. A 2000, 828, 133-139. (SCI) public NOV
8. Ming-Mu Hsieh, Yi-Chun Kuo, Pei-Ling Tasi , Huan-Tsung Chang*, “ Optimizing separation conditions for polycyclic aromatic hydrocarbons in micellar electrokinetic chromatography ”, J. Chromatogr. A 2001, 924, 397-405. (SCI) public JUY
9. 張煥宗、 黃銘峰、謝明穆、 黃志清“毛細管電泳暨雷射誘導螢光技術之發展與應用”科儀新知,民國九十年，第二十二卷，第五期，20-27頁。
10. Wei-Lung Tseng, Ming-Mu Hsieh, Shang-Ji Wang, Chih-Ching Huang, Yu-Cheng Lin,Po-Ling Chang, Huan-Tsung Chang*, “Analysis of large-volume DNA markers and polymerase chain reaction products by capillary electrophoresis in the presence ofelectroosmotic flow.”, J. Chromatogr. A. 2001,927 179-190. (SCI) public AUG
11. Chih-Ching Huang, Ming-Mu Hsieh, Tai-Chia Chiu, Yu-Cheng Lin, Huan-Tsung Chang*, “Mazimization of injection volumes for DNA analysis in capillary ectrophoresis” Electrophoresis 2001, 22, 4328-4332. (SCI) public DEC
12. Ming-Mu Hsieh, Chien-En Hsu, Wei-Lung Tseng, and Huan-Tsung Chang*, “Amplification of small analytes in polymer solution by capillary electrophoresis” Electrophoresis 2002, 23, 1633-1641. (SCI) public JUN
13. Ming-Mu Hsieh, Po-Ling Chang, Huan-Tsung Chang*,“ The impact of a plug of salts on the analysis of large volumes of ds DNA by capillary electrophoresis ” Electrophoresis 2002, 23, 2388-2393. (SCI) public AUG
14. Tai-Chia Chiu, Ming-Feng Huang,Chih-Ching Huang, Ming-Mu Hsieh, and Huan-Tsung*, “Indirect fluorescence of aliphatic carboxylic acids in non-aqueous capillary electrophoresi using merocyanine 540? Electrophoresis” 2002, 23, 445-449. (SCI) public FEB
15. 張煥宗、黃銘峰、謝明穆、黃志清” 毛細管電泳暨雷射誘導螢光技術之發展與應用” 2003年"中國科技發展精典文庫"下卷  2855-2856
16. Chung-Yi Chen, Shu-Ling Hsieh, Ming-Mu Hsieh, Sung-Fei Hsieh, Tian-Jye Hsieh” Substituent Chemical Shift of Rhammosides from the Stems of Cinnamomum osmophleum” Chin. Pharm. J. 2004. 56, 141-146. (SCI)
17. Huan-Tsung Chang Tai-Chia Chiu, Ming-Feng Huang, Chih-Ching Huang, Ming-Mu Hsieh“Advanced Capillary and Microchip Electrophoretic Techniques for Proteomics” Current Proteomics, 2004, 1,325-347. (SCI)
18. Yan-Wei Lin, Ming-Mu Hsieh, Ching-Piao Liu, and Huan-Tsung Chang*“ Photoassisted Synthesis of CdSe and Core-Shell CdSe/CdS Quantum Dots” Langmuir 2005, 21, 728-734 (SCI) public JAN
19. Ming-Mu Hsieh, Chang, H.-T.*” Discontinuous electrolyte systems for improved detection of biologically active amines and acids by capillary electrophoresis with laser-induced native fluorescence detection” Electrophoresis 2005, 26, 187–195.? (SCI). public JAN received June 9 2004, public Dec 29 2004
20. Tai-Chia Chiu, Shin-Huei Chiou, Ming-Mu Hsieh,Yit-Tsong Chen, and Huan-Tsung Chang*, “Photosynthesis of Gold Nanoparticles in Presence of Proteins” J. Nanosci. Nanotech. 2005, 5, 2128-2132. (SCI)
21. Ming-Mu Hsieh a , Tai-Chia Chiu b , Wei-Lung Tseng c and Huan-Tsung Chang , *“Analysis of Nucleic Acids and Proteins in Capillary Electrophoresis and Microchip CapillaryElectrophoresis Using Polymers as Additives of the Background Electrolytes”Current Analytical Chemistry, 2006, 2, 17-33. (SCI) IF 評估中
22. Ming-Mu Hsieh*, Huan-Tsung Chang, “Impacts of halides on the simultaneous separation of aromatic amines and their acidic metabolites by capillary electrophoresis with laser-induced native fluorescence detection under acidic conditions” J. Chromatogr. A. 2006, 1102,302-308. Received 27 June 2005; received in revised form 28 October 2005; accepted 1 November 2005 (January 13, 2006)
23. Yu-Fen Huang, Ming-Mu Hsieh, Wei-Lung Tseng, Huan-Tsung Chang*, “On-line Concentration of Microheterogeneous Proteins by Capillary Electrophoresis Using SDS and PEO as Additives” J. Proteomics 2006,5,429-436. (received September 5, 2005)
24. Ming-Mu Hsieh, Chung-Yi Chen, Shu-Ling Hsieh, Sung-Fei Hsieh, Pak-Hing Ben Lee, Cheng-Ta Li, ?Tian-Jye Hsieh*, “Separation of Phenols from the Leaves of Toona sinensis (Meliaceae) by Capillary Electrophoresis” J. Chin. Chem. Soc., 2006, 53, 1203-1208. (Received August 12, 2005.)
25. Cho-Chun Hu, Tai-Chia Chiu, Ming-Mu Hsieh,* and Huan-Tsung Chang,*, “Environmental Impact of Polynuclear Aromatic Hydrocarbons, Research Signpost”, 2007, in press.26. Ming-Mu Hsieh*, Sheng-Min Chen, “Determination of amino acids in tea leaves and beverages using capillary electrophoresis with light-emitting diode-induced fluorescence detection” Talanta, 2007, in press